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Subject: Re: SS-phenol?
Date: Mon, 1 Mar 2010 16:31:42 -0500
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Date: Mon, 1 Mar 2010 21:13:17 -0500
Reply-To: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
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From: Ralph Stuart <rstuart**At_Symbol_Here**UVM.EDU>
Subject: 15 RE: [DCHAS-L] SS-phenol?

=46rom the variety of responses, I clearly need to follow up with the lab tech to make sure I know what she has in mind. I wasn't sure if I was just out of the loop on a standard biochem abbreviation. It doesn't appear to be the case, but several of the responses below sound like what she has in mind, so I'll sound more intelligent when I talk to her.

Thanks to everyone who responded.

- Ralph
===

From: "Erik A. Talley" <ert2002**At_Symbol_Here**med.cornell.edu>
Date: March 1, 2010 4:31:35 PM EST
Subject: RE: [DCHAS-L] SS-phenol?

My staff say it's salt-saturated phenol...probably with sodium sulfite. I
did find some references to phenol/chloroform extractions using this mixture
of phenol, but I don't know the significance of the sodium sulfite.

Erik

===
From: Dan Herrick <herrickdan**At_Symbol_Here**hotmail.com>
Date: March 1, 2010 4:49:38 PM EST
Subject: RE: [DCHAS-L] SS-phenol?

Hi Ralph,

All I can think of is phenol red indicator solution, which is technically Phenol Red, Sodium Salt (CAS 34487-61-1) and they are abbreviating the Sodium Salt as SS?

I don't know of any chemistry abbreviations for (s,s) that would go with phenol.  

You could always have them bring you the bottle, I suppose.

Also...I hate to ask this but, if the lab tech's native language is not English, might they be trying to say something else?  It's been known to happen.

Hope you find out what it is.

Take care,

Dan Herrick
EHS Coordinator
Mechanical Engineering Department, MIT
herrickd**At_Symbol_Here**mit.edu
direct 617-253-2338

===
From: "Russell Vernon" <russell.vernon**At_Symbol_Here**ucr.edu>
Date: March 1, 2010 5:01:12 PM EST
Subject: RE: [DCHAS-L] SS-phenol?

Hi Ralph,
Taking that as a challenge I did some sleuthing

The choices seem to be solid state phenol (not real likely) and a formula such as:
700 mL Tris-HCl pH 8 saturated phenol. (ss-phenol)
To create a 'saturated phenol'

I'd be interested to see if you get an authoritative answer...

-Russ



Russell Vernon, Ph.D. 
russell.vernon**At_Symbol_Here**ucr.edu 
www.ehs.ucr.edu
(951) 827-5119


===
From: larry.e.wilkinson**At_Symbol_Here**exxonmo bil.com
Date: March 1, 2010 5:09:20 PM EST
Subject: Re: [DCHAS-L] SS-phenol?



Ralph,
I wonder if he/she is referring to the use of phenol-d6 as an "SS" (versus
an "IS" or internal standard) in an EPA GC method such as 8270?  I'd have
him/her bring you the container or give you the manufacturer and part
number so that you can see what chemical they are actually using.

Regards,
Larry

===
From: Evan A. Alberts [mailto:eaa2004**At_Symbol_Here**med.cornell.edu] 
Sent: Monday, March 01, 2010 4:32 PM
Subject: Phenol

 

Erik,

http://ambion.com/j p/techlib/spec/sp_9712.pdf

I believe the user information section of this MSDS answers the question of aqueous phases vs salt -saturated phases of phenol solutions and the various uses each is best for.

Hope this helps,

Evan

Evan Alberts, Environmental Specialist

Environmental Health and Safety

Weill Cornell Medical College

===
From: "Crouse William" <CrouseW1**At_Symbol_Here**wyeth.com>
Date: March 1, 2010 4:23:29 PM EST
Subject: Re: [DCHAS-L] SS-phenol?


It may refer to saturated phenol which in biological work is saturated with citrate buffer.

  

William E. Crouse, MS, CIH, CSP, CHMM
Director, Environmental Health and Safety
Pfizer Research
641 Ridge Road
Chazy, NY 12921-2420

 

518-846-6350 (office)
518-574-4457(pager)
518-846-6527 (fax)

 

===
From: "Karel Wright" <kwright**At_Symbol_Here**munzing.us>
Date: March 1, 2010 5:03:17 PM EST
Subject: RE: [DCHAS-L] SS-phenol?


Salt Saturated Phenol (SS phenol)
- Tris buffered Phenol pH 6.6/7.9
- 8-Hydroxyquionoline added until dark yellow/orange color

Detailed method of preparation described in Molecular and cellular
methods in developmental toxicology By George P. Daston.

http://books.google.com/books?id=Pl81b04T7lcC& printsec=frontcover&dq=%22
salt+saturated+phenol%22+preparation&source=gbs_book_othe r_versions_r&ca
d=10#v=onepage&q=&f=false

_________________________________
Karel Wright
Product Safety Manager
kwright**At_Symbol_Here**munzing.us

===
From: william parks <misterbill21225**At_Symbol_Here**yahoo.com> ;
Date: March 1, 2010 4:29:40 PM EST
Subject: Re: [DCHAS-L] SS-phenol?


I believe it means Salt-Saturated........................now, I could be wrong - lord knows that's happened before.

Hope this helps,

Bill Parks
CHST, CHMP, CEHT, LSP (C), RPIH
630/380-4032

**Providing sound Industrial Hygiene, Occupational Safety and Health, Environmental, and Laboratory support services and solutions for over 25 years**

===
From: "Brennan, Catherine (Environment Health & Safety)" <CRBRENNAN**At_Symbol_Here**ehs.unc.edu>
Date: March 1, 2010 4:31:15 PM EST
Subject: RE: [DCHAS-L] SS-phenol?

Buffer saturated phenol?  Not sure why it isn't called BS-phenol but maybe the SS stands for super saturated.
-Cathy


===
From: "Bush, Kimberly" <kimberly_bush**At_Symbol_Here**merck.com>
Date: March 1, 2010 4:32:18 PM EST
Subject: RE: [DCHAS-L] SS-phenol?


Is this supposedly a commercial chemical?  There's nothing by that name
in any of the chemical catalogs I have.  There are a number of
possibilities of what it could be, but they would all be pure
speculation with out a little more information.   

If you have any info about the chemical formula, chemical structure, or
source, I could narrow it down. 


Kimi Bush
Staff Chemist
MRLC S&E Committee Vice Chair
Merck & Co. Inc.
WP14-3
770 Sumneytown Pike
P.O. Box 4
West Point, PA 19486
ofc: 215-652-2766
cell: 610-223-9227
fax: 215-652-3971

===
From: "Kristi Ohr" <kohr**At_Symbol_Here**amherst.edu>
Date: March 1, 2010 4:35:02 PM EST
Subject: RE: [DCHAS-L] SS-phenol?


Hi Ralph,

It stands for salt saturated.  I believe it's just sodium chloride that's added.

Hope that helps,

Kristi

===
From: "Samuella B. Sigmann" <sigmannsb**At_Symbol_Here**appstate.edu>
Date: March 1, 2010 4:37:38 PM EST
Subject: Re: [DCHAS-L] SS-phenol?


"Stinky Stuff"
'Super Strong"

Sorry - I couldn't resist - Could it be Supersaturated?

===
From: "Eric Clark" <erclark**At_Symbol_Here**ph.lacounty.gov>
Date: March 1, 2010 4:41:39 PM EST
Subject: Re: [DCHAS-L] SS-phenol?


Perhaps sodium phenate - aka phenol sodium salt.  CAS 139-02-6.  

See also http://www.chemindustr y.com/apps/chemicals for other possible synonyms.   

Eric Clark, MS, CCHO, CHMM 
Safety & Compliance Officer 
Los Angeles County Public Health Lab  

===
From: "Gallagher, Kevin J." <Kevin.J.Gallagher**At_Symbol_Here**saint -gobain.com>
Date: March 1, 2010 5:14:55 PM EST
Subject: RE: [DCHAS-L] SS-phenol?


Ralph

Search supersaturated phenol

Kevin

Kevin J. Gallagher
Manager - Analytical Sciences
CertainTeed Insulation Group
1400 Union Meeting Road
Blue Bell, PA 19422-0761
215 274-2448 (direct)
610 766-1702 (mobile)
215 274-2515 (fax)


===
From: "Kohler, Chris E" <cekohler**At_Symbol_Here**indiana.edu>
Date: March 1, 2010 4:37:29 PM EST
Subject: RE: [DCHAS-L] SS-phenol?


I found a reference to "SS-Phenol" as a buffer-saturated-phenol used in plasmid separation. It says "it is prepared by addition of pure phenol to two or three volume of TE buffer in a separatory funnel. The mixture is shaken and let stand until the layers separate. The lower phenol layer is drained off. Repeat with fresh buffer until the phenol is neutralized (check with a pH meter). The resulting phenol is drained and stored at -20 C."

"Saturating the phenol with buffer prevents dehydration of the aqueous phase and loss of volume during extraction. The phenol denatures contaminating proteins and causes them to become insoluble and will accumulate at the interface between the lower phenol and upper aqueous phase."

Hope that helps.

Chris






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